To further verify this observation, we quantified the VEGF levels in the supernatant of DENV-1, -2, -3 and -4 infected HPMEC-ST1.6R cells at specific time points of 0, 8, 24, 96 and 192 hours post-infection using a microsphere-based immunoassay which utilized LuminexTM beads coupled to VEGF-specific antibodies as an analyte capture platform (BioPlex, Biorad, Hercules, California, USA), essentially as we had previously described [6]. This evidence concerns the gene VEGFA and infection.