Position β74 has also been shown to differentiate between the lower risk DRB1*0403 and *0406 T1D alleles, which contain a negatively charged glutamic acid (Glu), compared to the high risk DRB1*0401 allele which contain a non-charged polar Ala [23-26]. This evidence concerns the gene HLA-DRB1 and type 1 diabetes mellitus.