qRT-PCR was used to determine the expression levels of these two genes, and a small, less than 3-fold increase in the expression of both MuRF1 and Atrogin-1 was detected following induction of PrPC (Figure 3A and 3B); this is much lower than the 10–40 fold increase generally found in other models of muscle atrophy. This evidence concerns the gene FBXO32 and muscle atrophy.