To determine which integrin is required for the migratory and invasive properties of the melanoma cell lines, cells were treated with or without VN integrin-blocking antibodies [26,27] or IgG and their ability to invade and migrate through vitronectin-enriched Matrigel to the lower chamber of a Boyden chamber in the absence of serum or growth factors was evaluated. The gene discussed is VTN; the disease is melanoma.