On the other hand, most carcinomas (82 out of 91, 90%) exhibited methylation of at least one gene (Supplementary Figure 3A), with EN1 as the most frequently methylated gene in both carcinomas (66 out of 90, 73%) and adenomas (4 out of 10, 40%); SCTR also presented a high level of methylation in carcinomas (48 out of 90, 53%) and adenomas (3 out of 10, 33%); whereas INHBB showed the lowest rate of methylation (23 out of 91 carcinomas, 25%, and none of the 10 adenomas) (Figure 1 and Supplementary Table 4). This evidence concerns the gene EN1 and carcinoma.