To determine the role of nTregs in the development of lymphedema, we stimulated PBMCs from CP and INF patients with BmA or PPD for 24 h and examined the mRNA expression of nTreg markers—Foxp3, GITR, TGFβ, and CTLA-4—by real-time RT-PCR. The gene discussed is TNFRSF18; the disease is lymphedema.