To study the effect of Unc119 on bacterial infection, we knocked down Unc119 expression by 70–90% in human epithelial cells (Caco2 and HeLA) and mouse embryonic fibroblasts (NIH 3T3, abbreviated as 3T3) using siRNA (Figure 1A) and then infected them with Shigella flexneri. This evidence concerns the gene UNC119 and bacterial infectious disease.