In this paper, although no subjects with severe AATD deficiency were detected, we found six out of 120 subjects (5%) carrying the so called "intermediate deficiency", that means heterozygosity with one normal PI*M allele and one severe AATD allele [mean (SD) plasma AAT level: 0.93 g/L (0.12)]. Here, SERPINA1 is linked to alpha 1-antitrypsin deficiency.