In another approach utilizing different Extraction Buffers from a ProteoExtract® Subcellular Proteome Extraction Kit, we found the ≈120 and 65 kDa secreted PmpD post-translational products and the host cell cytoplasmic protein, GAPDH, in the material obtained at 48 h after infection with C. trachomatis serovar L2 which contains cytosolic proteins from the host cells and the chlamydial inclusion lumen (Extraction Buffer I) (Fig. 3). This evidence concerns the gene GAPDH and infection.