In the present study we wanted to study the potential of a heterologous prime boost vaccine strategy that induce both CD4 and CD8 T cells, and to compare the phenotype of vaccine primed CD4 and CD8 T cells, as well as the recruitment and proliferation of these cells following infection with M.tb. We combined vaccination of a CD4 T cell inducer, rH4/CAF01, with a strong CD8 T cell inducer, Ad-H4. The gene discussed is CD8A; the disease is infection.