Five of the eight variant alleles showed significantly higher allele frequency (CCL2 Insertion (I): p<0.0001; IL8 -251A: p<0.007; CCR5 Deletion (D): p<0.0001, CCR5 59029A: p<0.0001; MMP9 279Gln: p<0.0001) in DN as compared to DM group. This evidence concerns the gene MMP9 and diabetes mellitus.