CASP1 and infection: Whereas, in wild-type macrophages, greater than 60% of the phagosomes containing the pathogen co-localized with LAMP-1 within 30 min post-infection, less than 30% of the L. pneumophila containing vacuoles in caspase-7−/−, caspase-1−/− and A/J-derived macrophages acquired LAMP-1 (Figure 4A and 4B).