The central novel findings of our study are: a), the identification of two separate regions within the 5′ flanking region of mammalian fascin-1 genes in which specific, highly conserved sequence motifs are concentrated; b), the identification of a combined action of the well-conserved CREB- and AhR-binding motifs in positive transcriptional regulation of FSCN1, and c), the identification that AhR and CREB transcription factors are specifically associated with the FSCN1 promoter in fascin-positive human colon carcinoma cells. Here, CREB1 is linked to colon carcinoma.