To further explore the effect of CCL20 overexpression on prostate cancer cell behavior, we assessed the adhesion of PC3 clones that overexpress CCL20 to ECM proteins fibronectin and collagen I. CCL20-overexpressing PC3 cells were grown to confluence, harvested, and allowed to adhere to fibronectin or collagen I-coated plates. Here, FN1 is linked to prostate cancer.