To further investigate the viral infection procedure, we used flow cytometry to quantitatively determine if an MOI of 50 would result in similar levels of infection for all three AAVs (AAV‐CaM‐peptide + GFP, AAV‐scram‐CaM‐peptide + GFP or AAV‐GFP) in differentiated SH‐SY5Y cells not expressing mutant huntingtin (non‐htt‐SHSY5Y cells) and in differentiated SH‐SY5Y cells that stably express mutant huntingtin (SHSY5Y‐htt‐N63‐148Q cells). The gene discussed is HTT; the disease is infection.