To further investigate the viral infection procedure, we used flow cytometry to quantitatively determine if an MOI of 50 would result in similar levels of infection for all three AAVs (AAV‐CaM‐peptide + GFP, AAV‐scram‐CaM‐peptide + GFP or AAV‐GFP) in differentiated SH‐SY5Y cells not expressing mutant huntingtin (non‐htt‐SHSY5Y cells) and in differentiated SH‐SY5Y cells that stably express mutant huntingtin (SHSY5Y‐htt‐N63‐148Q cells). Here, HTT is linked to viral infectious disease.