By comparing luciferase levels in A375N melanoma cells that overexpress SPARC compared to breast cancer T-47D and cervical cancer HeLa cell lines that exhibited very low levels of SPARC (see Table 1 for relative SPARC mRNA levels in different cell lines), we selected the −513/+35 fragment, and named it F512Pr (Figure 1A). The gene discussed is SPARC; the disease is breast cancer.