NOS2 and central nervous system cancer: To determine if SUMOylation was involved in inflammatory activation, rat C6 glioma cells stably transfected with luciferase reporter constructs pNFκB (4 × NFκB binding-element) or pGL3-2.2 (containing the 2.2 κB NOS2 promoter sequence)were transiently transfected with SRG expression plasmids and the promoter activation by LPS was determined (Figure 3).