The levels of Ser473 phosphorylated PKB/Akt measured by ELISA showed considerable inter-tumoural heterogeneity, which reflects the complex nature of these tumours relative to subcutaneous implants of cell lines, but there was an obvious decrease in the mean values in all five models at 2 h, followed by recovery over 24 h (Figure 3). Here, AKT1 is linked to neoplasm.