In the four lobular carcinomas evaluated by all three methodologies (CISH, qRT-PCR, and IHC), two presented discordant results: one case showed high-level amplification by CISH, HER-2 overexpression by qRT-PCR and negative immunostaining; the other sample presented nonamplified by CISH, RQ ≤ 2.00 by qRT-PCR and 2+ immunostaining by IHC analysis. This evidence concerns the gene ERBB2 and breast lobular carcinoma.