Another human colon cancer cell line adapted to LF conditions, WiDr LF, displayed only a moderate increase in the constitutive expression of BCRP protein and mRNA levels (1.6-fold) compared with their HF counterpart, as revealed by western blot and real-time LC-PCR (Figure 1A and B). The gene discussed is ABCG2; the disease is malignant colon neoplasm.