First of all, we did antiviral state assay in vitro by pretreating MEFs cells from wild-type, STAT1KO and P117 mice with 2-fold serial dilution of IFNα starting from 1000 U/ml followed by EMCV infection at M.O.I. of 0.1 and scored the lytic activity in MEFs. The gene discussed is IFNA1; the disease is infection.