ANXA5 and acute myeloid leukemia: As expected no apoptosis was observed with flow cytometric analysis of annexin V FITC and propidium iodide staining (P1, P3) during the first 6 hours of treatment (Figure 1B), and examination of AML blasts for cell shrinkage or changes in nuclear morphology within 24 h after start of treatment revealed no signs of apoptosis (data not shown).