To corroborate the DCIS-specific methylation profiles of TLX1, CGI 7:48, HOXB13, and HNF1B, we then extended the COBRA analysis to a series of primary breast tumors of different histological type and stage (Figures 3 and 5, lower panels and Figure 4). The gene discussed is HOXB13; the disease is ductal breast carcinoma in situ.