NR2E1 and ductal breast carcinoma in situ: In agreement with the MIRA results, we found that the GFI1 CpG island was hypermethylated in 14 out of the 18 tumors examined (six DCIS and 12 stage I tumors, 78%) while the NR2E1 target region was methylated in 11 out of the 21 early-stage breast tumors (six DCIS and 15 stage I tumors, 52%) (Figure 4, partial data and Table 3).