Moreover, similar amounts were detected in two different cell lines derived from colon carcinoma (HCT116 p53+/+ and -/-), and in non-cancerous cells such as MRC-5 fibroblasts and foreskin fibroblasts (Figure 1A), indicating that NEDD1 expression is not strongly deregulated in cancer cells. This evidence concerns the gene NEDD1 and colon carcinoma.