First, we examined the mRNA expression levels of KIF5B in three cancer cell lines(MCF-7, HeLa and U2OS osteosarcoma) and found it to be highly expressed in allthree cell lines when compared to other N-kinesins including KIF5A/KIF5C(Kinesin 1), KIF3A (Kinesin 2) and KIF1A (Kinesin 3) (Fig. 1B). This evidence concerns the gene KIF5C and osteosarcoma.