Monospecificity of antibodies against Mcm2, geminin, Plk1, Aurora A and the Aurora kinase substrate H3S10ph was confirmed in total cell extracts from asynchronous MCF-7 breast cancer cells by detection of a single protein with a molecular mass consistent with the reported electrophoretic mobility of the corresponding human antigen (Supplementary Figure 1A). This evidence concerns the gene MCM2 and breast carcinoma.