Previous investigators, although often not including all four subtypes in the analysis, have in general found that expression of CK7, MUC1 and MUC4 predominantly identifies tumours with pancreatobiliary differentiation and a poor prognosis, whereas expression of CK20, MUC2 and CDX2 predominantly identifies tumours with intestinal differentiation and a good prognosis.6,8,17,18,21–25,39 No particular combination of biomarkers has been established for the immunohistochemical classification of histological type. This evidence concerns the gene MUC4 and neoplasm.