These results, confirmed at the mRNA level by Real-Time PCR (Figure 6A, B, right hand panels), are consistent with the notion that the un- and hypomethylated promoters of these two genes are suppressed in melanoma cells by acetylated histone H3, and that Aza can release HDAC1 suppression and can act in synergy with HDAC inhibitors, as reported for AML and colorectal carcinoma cells [37]. The gene discussed is HDAC1; the disease is melanoma.