However, since the use of BLTs could not unambiguouslydemonstrate that HDL-mediated infection-enhancement requires SR-BI-dependentlipid transfer, next, we expressed and analyzed effects of SR-BI ectodomainmutants E418R, Q402R and Q402R/E418R that have reduced lipid uptake properties[52],[54], as shown in Figure 5D. This evidence concerns the gene SCARB1 and infection.