Therefore to investigate the possibility that oxidative stress could be influencing the release of MCP-1, the stably transfected SH-SY5Y cells were treated for 48 hours with Trolox, a water-soluble form of vitamin E. Additionally, cells were treated with minocycline, an antibiotic with iron chelation, antioxidant, anti-inflammatory, and mitochondria-protective properties [18-21], because this drug has been explored for the treatment of neurodegenerative diseases. The gene discussed is CCL2; the disease is neurodegenerative disease.