Sequencing analysis revealed six novel missense mutations (p.F55L, p.P132L, p.S136G, p.C149Y, p.D203Y, and p.H249R), one nonsense mutation (p.S48X), one frame shift (p.P297fs), and three previously reported missense mutations (p.P31L [13], p.C165Y [14], and p.R127C [15]) in CHST6 in the Iranian patients with MCD. This evidence concerns the gene CHST6 and macular corneal dystrophy.