Therefore, it is of significance that, in this investigative study, we have developed a relatively simple, high throughput assay based on methylation of a sequence in the promoter of the ACSL3 gene that appears to distinguish children born to mothers with high airborne PAH exposure from those who were not (OR = 13.8; p<0.001) with high sensitivity (75%) and specificity (85%). This evidence concerns the gene ACSL3 and pulmonary arterial hypertension.