However, with the exception of one inoculum, I026, where two mice were not affected, all sporadic and iatrogenic CJD-inoculated mice were scored as positive for prion infection by one or more of the following criteria: typical clinical signs, presence of PrPSc on Western blot analysis or abnormal PrP immunohistochemistry (Table 2). Here, PRNP is linked to Creutzfeldt Jacob disease.