In this research, we used cultured neonatal ventricular myocytes as a model to study the influence of high glucose levels on the structure, function and expression of PKC, NF-κB, TNF-α and c-fos in cardiomyocytes, and tried to study the effect of PKC/NF-κB/c-fos signal transduction pathway in the pathogenesis of diabetic cardiomyopathy. This evidence concerns the gene PRRT2 and diabetic cardiomyopathy.