DAG1 and Hyperglycemia: Over-expression of PKC-α and PKC-β2 were discovered in the early stage of pathological development of diabetic cardiomyopathy because sustained hyperglycemia in diabetes mellitus could activate DAG-PKC signal transduction pathway, leading to increased expression of PKC in cardiomyocytes and translocation of PKC from cytoplasm to cytomembrane[31-33].