We also noted that MP-12 co-infection did not reduce the abundance of the viral-sense rMP-12-rLuc S segment in the presence of ActD, and yet it caused the reduction of rLuc mRNA abundance (Figure 3B, lane 9), which we believe implies that NSs expression and ActD treatment generated a cellular environment that was more favorable for rMP-12-rLuc RNA replication than for transcription. The gene discussed is SPRR2A; the disease is coinfection.