This finding comes inagreement with previous data suggesting increased levels of soluble CD25 and upregulationof CD4+/CD25+ T-cells in acutely ill brucellosis patients [21, 37, 38].Generally, previous in vitro and in vivo human studies showed that LPSstimulation of normal T-lymphocytes upregulates the CD25 expression and thesecretion of IL-2 and IFNγ bythem. This evidence concerns the gene CD4 and brucellosis.