From the breast cancer list we selected two new control genes which were validated in an RT-QPCR assay that also included five previously used control genes (ACTB, TFRC, GUSB, RPLP0 and GAPDH – see [17]) and breast cancer-related genes (e.g. ESR1, ERBB2, AURKA, etc.). This evidence concerns the gene ESR1 and breast carcinoma.