Co-IP confirmed that the CRP∶L-ficolin complex formation was induced in the serum by infection-inflammation condition (low pH, low calcium level and high CRP level) (Figure 2A and 2B) and ELISA demonstrated that L- and M- ficolins displayed stronger binding to CRP when the latter was anchored first to bacteria (Figure 2C and 2D). Here, FCN1 is linked to infection.