While all HIV-1 infections with the analyzed Nef mutants displayed apparently intermediate p24 production (Fig. 4A), this difference only reached low statistical significance for NefLLAA and NefΔ12–39 (Δnef p = 0.0017, G2A p = 0.16, E4A4 p = 0.19, AxxA p = 0.12, LLAA p = 0.03, KKAA p = 0.33, Δ12–39 p = 0.026). The gene discussed is S100B; the disease is HIV-1 infection.