Our data did not reveal any correlation between hypoxia induced HIF-1α protein over expression and GAPDH regulation on mRNA and protein level in vitro in Hep-1-6 mouse hepatoma, Hep-3-B and HepG2 human hepatocellular carcinoma cell lines as well as in human lung adenocarcinoma epithelial cell line (A-549), in addition to both HT-29, and. This evidence concerns the gene GAPDH and lung adenocarcinoma.