TKT and neoplasm: DC activation in response to GL26 tumor extracts was dependent on TLR2 expression; we observed that the release of the pro-inflammatory cytokines TNFα and IL6 was elevated when WT BMDC were incubated with either media from GL26 cells killed with Ad-TK and GCV or necrotic GL26 cells (*, p < 0.05 versus mock; Figure 6C, η2 = 1.0; Figure 6D, η2 = 0.73).