Also, increased levels of HMGB1 in the serum of mice bearing GL261 or B16-F10 tumors after treatment with Ad-Flt3L and Ad-TK indicate that HMGB1 release is not limited to tumor derived from GL26 cells, and in fact, is released from tumors originating from various other cell types. The gene discussed is FLT3LG; the disease is neoplasm.