Figure 9A–9D reveals statistically significant increases (*p < 0.05 versus mock and Ad0) in HMGB1 levels in the supernatants of GL26 (η2 = 0.67), LLc1 (η2 = 0.68), GL261 (η2 = 0.73), or B16-F10 cells (η2 = 0.72) in response to Ad-TK (+GCV). Irradiation (Figure 9E–9H) and temozolomide (Figure 9I–9L) treatments also caused a significant increase (*, p < 0.05) in the levels of HMGB1 released from GL26 (η2 = 0.60 and 0.63, respectively), LLc1 (η2 = 0.60 for both treatments), GL261 (η2 = 0.60 for both treatments), and B16-F10 tumor cells (η2 = 0.67 and 0.60, respectively). Here, HMGB1 is linked to neoplasm.