We found that 15% of these samples had anti-O antibody titers of ≥ 1:80 and 16% had anti-H antibody titers of ≥ 1:160 against Salmonella enterica serotype Typhi. In Nepal, the current reference baseline titer of Widal agglutination test for diagnosis of typhoid fever is 1:80 for both O & H agglutinins. Here, FUT1 is linked to typhoid fever.