The expression levels of MUC4 mRNA in the human breast cancer cell lines MCF-7, T-47D and MDA-MB-453, the human lung cancer cell lines NCI-H292 and A427, the human pancreatic carcinoma cell lines HPAFII, BxPC-3 and PANC1 and the human colon adenocarcinoma cell lines LS174T and Caco2 were examined using quantitative RT–PCR analysis (Figure 1A). The gene discussed is MUC4; the disease is breast carcinoma.