3A–3B). Higher concentrations of cilengitide abolished cell proliferation and induced apoptosis already after 24 hours exposure to the drug. Annexin V/propidium iodide staining revealed the presence of 18% apoptotic G28 and 30% apoptotic G44 cells after treatment with 5 μg/ml cilengitide. At a concentration of 50 μg/ml, 35% – 50% of G28 and G44 cells respectively underwent apoptosis showing a dose dependent apoptosis induction in glioma cells (fig. 3C–3D). This evidence concerns the gene ANXA5 and central nervous system cancer.