Both OVCA 433 and SKOV3 cell lines produced IL-6 endogenously but, in the presence of EGF, the production of IL-6 was increased (P<0.05) in the serum-free medium of both ovarian cancer cell lines (Figure 4B), consistent with the mRNA enhancement of IL-6 and IL-6Rα on the cells (Figure 4A). This evidence concerns the gene EGF and ovarian carcinoma.