Whereas Smad3 gene expression in COPD stage II fibroblasts was significantly downregulated at 1- and 24-hour stimulation (figures 2 and 3; additional file 1), Smad4 and Smad7 gene expression was upregulated, Smad2 was upregulated only at 24 hours. This evidence concerns the gene SMAD3 and chronic obstructive pulmonary disease.