Since it is difficult to culture and maintain primary murine IECs, we depleted Kindlin-1 in a human colon carcinoma cell line (HT-29) using RNAi (HT-29siKind1; Figure 8B) to show that integrin-mediated cell adhesion and shear stress induced detachment were also perturbed in a colon cell line. Here, FERMT1 is linked to colon carcinoma.