We treated human hepatoma cell line, HepG2 cells, with elevated physiological level (0.7 mM) of different types of FFAs (saturated, monounsaturated and polyunsaturated) “crossed” with 3 different levels of TNF-α, 0, 20 and 100 ng/ml for 24 h. The gene discussed is TNF; the disease is hepatocellular carcinoma.