In total, we had a conclusion that the dual small interfering RNA expression system targeting to MAT2A and MAT2β simultaneously was constructed successfully, LV-siMAT2A/2β can lead to grow-inhibition and cell apoptosis which is more than LV-siMAT2A and LV-siMAT2β, this may be a new way for the therapy of hepatic cancer in the future. The gene discussed is MAT2B; the disease is liver cancer.