The fourth missense mutation (R346T), identified in a Spanish patient with POAG [30] was considered as an example of a myocilin variant involved in non-Mendelian glaucoma, whereas the nonsense mutation Q368X has been found in both autosomic dominant juvenile-onset glaucoma and non-Mendelian cases of glaucoma [5].The HEK-293T cell line was chosen because is well characterized, allows easy expression of recombinant proteins and has been previously used as a cellular model to study different recombinant human proteins including myocilin [9,25,31,32]. This evidence concerns the gene MYOC and open-angle glaucoma.